The gram stain the gram stain is the most frequently used stain in a clinical microbiology laboratory and is usually the first step in identifying bacteria based on differences in cell wall components, bacteria are categorized as either gram- positive (stains dark purple) or gram-negative (stains pink) in addition to the gram. Medical microbiology experiment 1 wang yuyan bacterial stain living bacteria stain killed bacteria stain positive staining negative staining common stain single-dye stain special stain complex stain endospore stain capsule stain gram stain cell wall stain acid fast stain methods for staining bacteria. View lab report - micro lab report 1 from micro 3050 at clemson lab 1: the gram stain china moore instructor: nattasha vinas microbiology 3051 sec 8 february 3, 2015 this report represents my. The gram stain, originally developed in 1884 by christian gram, is probably the most important procedure in all of microbiology it has to be one of the most repeated procedures done in any lab gram was actually using dyes on human cells, and found that bacteria preferentially bind some dyes the gram stain is a. Lisa ta february 29, 2012 biology 240 general microbiology mw 3:45-5:00 gram stain report introduction the purpose of this experiment is to determine the shape and gram stain of the bacteria under a microscope the reason for staining bacteria is due to the fact that most bacteria are transparent and cannot be. The gram stain is a classical and important staining technique that remains widely used by environmental microbiologists similar to a simple stain, it allows collect soil sample and transport to the laboratory for microbial analysis in the lab, weigh a 10 g sample using an analytical balance dilute the sample 1:10 into 95.
View lab report - differential staining lab report revised from scibls 101 at nc state harrington 1 differential staining of escherichia coli and staphylococcus epidermidis using the gram stain. In this lab you will be innoculating plates and observing bacterial growth microscopes can then be used to identify specific bacteria this lab may take several days, keep all data and observations in a separate notebook to be compiled and organized into a final lab report. Gram positive bacilli that are pleomorphic, club shaped, or irregularly staining or that have palisading and/or angular arrangements (v and l shapes) branching or cellular extensions recording observations each laboratory must have sops (standard operating procedures) for reporting. The bacteria growth was assessed visually by colony counting and bacterial morphology positive samples were selected and bacteria gram stain was performed the morphology of bacteria was observed and captured by a nikon e200 microscope system table 1 sample information for the experiment brand name.
A gram stain the previous lab introduced simple staining techniques that enable microbiologists to observe the morphological characteristics of bacteria although simple stains are useful, they each student will have the opportunity to submit one example of a gram stain with a brief written report describing the staining. .
The preparation of a smear is required for many laboratory procedures, including the gram-stain the purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure a smear can be prepared from a solid or broth medium below are some guidelines for. Introduction gram staining is the most essential and universally used staining technique in bacteriology laboratory gram-staining was firstly introduced by cristian gram in 1883this method is used to distinguish between gram positive and gram-negative bacteria which have consistent differences in their cell walls. A video showing the gram stain technique for visualising bacteria this ver plus, my lab instructor knew that people were struggling with it so we've been doing nothing but gram stains thanks for i can only hope i'll be able to properly perform a gram stain by the time my lab practicum comes around. Introduction gram staining is the most essential and universally used staining technique in bacteriology laboratory sandiford's modification of gram stain in gram staining bacteria fixed to a slide are treated the gram staining method is named after the danish bacteriologist 07-14-10, lab report 10- 2-10,.
Has your laboratory ever had difficulty correctly reporting out cerebrospinal and joint fluid gram stains during off hours this course helps provide training for technologists who must read gram stain but do not work primarily in the microbiology laboratory great for cross-training and for clinical laboratory science students as. Gram stain is one of the most common staining procedures it is one of the differential stains used to characterize bacteria as either gram positive bacteria or gram negative bacteria. Microbiology lab report : 1- introduction (gram staining) 2-requirements ( reagents) 3-method 4-observations college of medicine al-imam mohammad bin saud.
A gram stain is a lab test used to detect bacteria or fungi in a sample taken from the site of a suspected infection it gives relatively quick, preliminary results as to whether microbes are present and, if so, the general type(s) causing an infection. Student procedures purpose: is to determine the gram stain of your bacterial sample introduction: gram staining is a method commonly used to determine the chemical make up of the cell wall of bacteria the cell wall can stain either positive or negative, depending on its chemistry knowing the chemical make up makes it. Abstract we assessed the usefulness of reporting direct blood gram stain results compared with the results of positive blood cultures in 482 episodes and moni the clinical microbiology laboratory has an important role in the management of bacteremia detecting pathogenic microorganisms in blood.
Hypothesis here is where the purpose of the lab is summarized in the hypothesis statement the question(s) to be answered during the lab is (are) clearly identified and stated using an 'ifthen' statement(s) if i apply the gram stain sequence to an air dried/heat fixed slide of bacteria then i may observe__________. Gram staining: principle, procedure, interpretation, examples and animation gram staining is the common, important, and most used differential staining techniques in microbiology, which was introduced by danish bacteriologist hans christian gram in 1884 this test differentiate the bacteria into gram. A common occurrence is to observe organisms present in the gram stain (eg, gram-positive bacilli, gram-positive cocci, or gram-negative bacilli) and to report these on subculture, the organisms do not grow these false-positive reports of organisms present a challenge to the laboratory liquid nutrient media such as. Detailed principle, reagent preparation, procedure, result interpretation, examples and animation video for gram staining technique it is typically the first differential test run on a specimen brought into the laboratory for identification in some cases, a rapid, presumptive identification of the organism or.